miRNAs associated to endoplasmic reticulum stress and unfolded protein response during decidualization

Research question: Do miRNAs play a role in regulating endoplasmic Reticulum Stress (RS) and Unfolded Protein Response (UPR) decidualized cells endometrium associated with reproductive failures?Design: Endometrial stromal cell line St-T1b was vitro 8-Br-cAMP during 5 days, or treated the RS-inducer Thapsigargin (Tg). Expression of RS-sensors, UPR markers potential miRNA regulators were analysed by qPCR. biopsies from patients recurrent pregnancy loss (RPL) implantation failures (RIF) investigated for location expression.Results: After decidualization, increased expression RS-sensors including ATF6α, PERK, IRE1α, marker, CHOP. TXNIP, which serves as link between RS-pathway inflammation, well inflammasome NLRP3 IL-1β cells. An silico analysis identified miR-17-5p, miR-21-5p, miR-193b-3p potentially involved regulation RS/UPR pathways inflammation embryo implantation. Their significantly decreased non-decidualized presence Tg. Finally, selected localized situ hybridization glandular epithelial endometrial samples RPL RIF patients. stroma lower comparison those patients.Conclusions: Decidualization is accompanied processes an inflammatory response that influenced miR-193b-3p. These are differentially expressed indicating alteration pathways.